T6S | Multi-scale model of bacterial cell-cell interactions

Summary
Transporting selected proteins across the cell envelope in a controlled manner is a fundamental property of all cell types. For bacteria, secretion systems play a central role in the translocation of effectors that mediate interactions with other cells. The bacterial Type VI Secretion (T6S) system is one of the most widespread secretion systems and, in a cell-cell contact-dependent fashion, it injects effectors into eukaryotic and bacterial targets. The T6S system utilizes a cytoplasmic phage tail-like apparatus that is anchored to the cell envelope. A contractile sheath propels a macromolecular effector-loaded tube/spike-complex outside the cell and into the target.

Here, we will establish a model of T6S-mediated cell-cell interactions by integrating information from the molecular, cellular and intercellular scales. The model will reveal answers to three paramount questions of the field: Aim 1 (molecular scale) will elucidate an atomic model of the T6S membrane-anchoring complex in loaded and fired conformational states. Aim 2 (cellular scale) will identify the mechanisms and relevance of clustering multiple T6S machines in a confined region of the cell. Aim 3 (intercellular scale) will resolve T6S actively engaged in cell-cell interactions to clarify the in vivo structure and significance of the inner tube in effector translocation and target cell penetration.

Electron cryo-tomography (ECT) will be used as the key technique to resolve unique structures in their cellular context, in a native state, in three dimensions and at the crucial nanometer-to-micrometer range. Cryo-focused ion beam milling will be established to prepare samples thin enough for ECT imaging. ECT data will be complemented by high-resolution structural information of sub-complexes (in vitro) and low-resolution fluorescence light microscopy data on dynamics (in vivo). In the future, our approach will prove useful to study other types of bacterial cell-cell interactions.
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More information & hyperlinks
Web resources: https://cordis.europa.eu/project/id/679209
Start date: 01-01-2017
End date: 31-12-2021
Total budget - Public funding: 1 751 853,00 Euro - 1 751 853,00 Euro
Cordis data

Original description

Transporting selected proteins across the cell envelope in a controlled manner is a fundamental property of all cell types. For bacteria, secretion systems play a central role in the translocation of effectors that mediate interactions with other cells. The bacterial Type VI Secretion (T6S) system is one of the most widespread secretion systems and, in a cell-cell contact-dependent fashion, it injects effectors into eukaryotic and bacterial targets. The T6S system utilizes a cytoplasmic phage tail-like apparatus that is anchored to the cell envelope. A contractile sheath propels a macromolecular effector-loaded tube/spike-complex outside the cell and into the target.

Here, we will establish a model of T6S-mediated cell-cell interactions by integrating information from the molecular, cellular and intercellular scales. The model will reveal answers to three paramount questions of the field: Aim 1 (molecular scale) will elucidate an atomic model of the T6S membrane-anchoring complex in loaded and fired conformational states. Aim 2 (cellular scale) will identify the mechanisms and relevance of clustering multiple T6S machines in a confined region of the cell. Aim 3 (intercellular scale) will resolve T6S actively engaged in cell-cell interactions to clarify the in vivo structure and significance of the inner tube in effector translocation and target cell penetration.

Electron cryo-tomography (ECT) will be used as the key technique to resolve unique structures in their cellular context, in a native state, in three dimensions and at the crucial nanometer-to-micrometer range. Cryo-focused ion beam milling will be established to prepare samples thin enough for ECT imaging. ECT data will be complemented by high-resolution structural information of sub-complexes (in vitro) and low-resolution fluorescence light microscopy data on dynamics (in vivo). In the future, our approach will prove useful to study other types of bacterial cell-cell interactions.

Status

CLOSED

Call topic

ERC-StG-2015

Update Date

27-04-2024
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Horizon 2020
H2020-EU.1. EXCELLENT SCIENCE
H2020-EU.1.1. EXCELLENT SCIENCE - European Research Council (ERC)
ERC-2015
ERC-2015-STG
ERC-StG-2015 ERC Starting Grant