Summary
Anionic lipids are critical for membrane organization and signaling in eukaryotes, including animals and plants. The dogma is that they accumulate in distinct membranes, thereby recruiting a unique set of lipid-binding proteins to each compartment. In turn, these proteins regulate trafficking and signaling activities on these compartments. However, it is increasingly recognized that anionic lipid distribution is not organelle-specific. In particular, we uncovered the presence of anionic lipids concentration gradients between the membranes of various compartments in plant cells. We further found that these cellular lipid gradients are dynamically regulated during cell differentiation and rapid responses to auxin, one of the major regulators of plant growth and architecture.
Here, we hypothesize that lipid gradients act as a nexus between signaling and trafficking regulations, and thus are critical yet unstudied factors coordinating the very dynamics of the plant endomembrane network.
To test this hypothesis, we will induce controlled fluctuations in anionic lipid gradients to analyze their direct impact on endomembrane morphodynamics, intracellular trafficking and hormone signaling across a broad range of scales ranging from their nano-organization to multicellular development. To do this, we will deploy a portfolio of complementary and innovative approaches, such as optogenetics, subcellular proteomics, and super-resolution live imaging, to visualize and perturb anionic lipids in planta and, for the first time, at relevant spatiotemporal scales.
LIPIDEV represents a complete change of perspective by 1) hypothesizing the importance of lipid gradients in plant cell functions, 2) considering these gradients in their multicellular context, 3) addressing the function of specific lipid pools within cells and tissues, and 4) changing the time scale at which we study anionic lipids, allowing us to dissociate direct and indirect effects associated with these lipids.
Here, we hypothesize that lipid gradients act as a nexus between signaling and trafficking regulations, and thus are critical yet unstudied factors coordinating the very dynamics of the plant endomembrane network.
To test this hypothesis, we will induce controlled fluctuations in anionic lipid gradients to analyze their direct impact on endomembrane morphodynamics, intracellular trafficking and hormone signaling across a broad range of scales ranging from their nano-organization to multicellular development. To do this, we will deploy a portfolio of complementary and innovative approaches, such as optogenetics, subcellular proteomics, and super-resolution live imaging, to visualize and perturb anionic lipids in planta and, for the first time, at relevant spatiotemporal scales.
LIPIDEV represents a complete change of perspective by 1) hypothesizing the importance of lipid gradients in plant cell functions, 2) considering these gradients in their multicellular context, 3) addressing the function of specific lipid pools within cells and tissues, and 4) changing the time scale at which we study anionic lipids, allowing us to dissociate direct and indirect effects associated with these lipids.
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| Web resources: | https://cordis.europa.eu/project/id/101001097 |
| Start date: | 01-10-2021 |
| End date: | 30-09-2026 |
| Total budget - Public funding: | 2 374 844,00 Euro - 2 374 844,00 Euro |
Cordis data
Original description
Anionic lipids are critical for membrane organization and signaling in eukaryotes, including animals and plants. The dogma is that they accumulate in distinct membranes, thereby recruiting a unique set of lipid-binding proteins to each compartment. In turn, these proteins regulate trafficking and signaling activities on these compartments. However, it is increasingly recognized that anionic lipid distribution is not organelle-specific. In particular, we uncovered the presence of anionic lipids concentration gradients between the membranes of various compartments in plant cells. We further found that these cellular lipid gradients are dynamically regulated during cell differentiation and rapid responses to auxin, one of the major regulators of plant growth and architecture.Here, we hypothesize that lipid gradients act as a nexus between signaling and trafficking regulations, and thus are critical yet unstudied factors coordinating the very dynamics of the plant endomembrane network.
To test this hypothesis, we will induce controlled fluctuations in anionic lipid gradients to analyze their direct impact on endomembrane morphodynamics, intracellular trafficking and hormone signaling across a broad range of scales ranging from their nano-organization to multicellular development. To do this, we will deploy a portfolio of complementary and innovative approaches, such as optogenetics, subcellular proteomics, and super-resolution live imaging, to visualize and perturb anionic lipids in planta and, for the first time, at relevant spatiotemporal scales.
LIPIDEV represents a complete change of perspective by 1) hypothesizing the importance of lipid gradients in plant cell functions, 2) considering these gradients in their multicellular context, 3) addressing the function of specific lipid pools within cells and tissues, and 4) changing the time scale at which we study anionic lipids, allowing us to dissociate direct and indirect effects associated with these lipids.
Status
SIGNEDCall topic
ERC-2020-COGUpdate Date
27-04-2024
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