Summary
Recent encouraging clinical results obtained with engineered T lymphocytes and increasing advances in the genome editing
field, have opened new opportunities for T-cell receptor (TCR) gene therapy as an immunotherapeutic approach for cancer.
Unfortunately, the broad applicability of this treatment is still hampered by the possible mispairing of exogenous/endogenous
TCR chains and by the limited number of high avidity tumor-specific TCRs. While the first issue has been successfully
addressed by the hosting lab with the development a TCR gene editing protocol, the identification of novel tumor-specific
TCRs is urgently required and this is the aim of my research proposal. We have the unique opportunity to combine the highly
complementary expertise of the hosting lab in T-cell biology/genetic transfer and of the applicant on immune repertoire
sequencing. We will target acute myeloid leukemia (AML) and hypothesize that by exploiting intrinsic features of AML (i.e.
ability of AML blasts to differentiate into potent antigen presenting cells expressing tumor antigens), the functional fingerprint
induced by AML on tumor-reactive T-cells, and cutting-edge technologies (i.e. next generation sequencing; ligandome
landscape analysis), we will provide a comprehensive immunoprofiling of tumor-specific T-cells and isolate tumor TCR
specificities. Results obtained in this study will streamline TCR hunting studies in solid tumors, leading to the generation of a
TCR library for different antigens and HLA restrictions, thus rendering TCR gene editing an innovative off-the-shelf
treatment available for a high number of cancer patients. Awarding this fellowship will greatly enhance researcher’s career
not only by providing the opportunity to widen scientific knowhow and acquire new skills, but also by enabling the researcher
to address a major bottleneck currently limiting the full exploitation of the rapidly growing field of cancer immunotherapy.
field, have opened new opportunities for T-cell receptor (TCR) gene therapy as an immunotherapeutic approach for cancer.
Unfortunately, the broad applicability of this treatment is still hampered by the possible mispairing of exogenous/endogenous
TCR chains and by the limited number of high avidity tumor-specific TCRs. While the first issue has been successfully
addressed by the hosting lab with the development a TCR gene editing protocol, the identification of novel tumor-specific
TCRs is urgently required and this is the aim of my research proposal. We have the unique opportunity to combine the highly
complementary expertise of the hosting lab in T-cell biology/genetic transfer and of the applicant on immune repertoire
sequencing. We will target acute myeloid leukemia (AML) and hypothesize that by exploiting intrinsic features of AML (i.e.
ability of AML blasts to differentiate into potent antigen presenting cells expressing tumor antigens), the functional fingerprint
induced by AML on tumor-reactive T-cells, and cutting-edge technologies (i.e. next generation sequencing; ligandome
landscape analysis), we will provide a comprehensive immunoprofiling of tumor-specific T-cells and isolate tumor TCR
specificities. Results obtained in this study will streamline TCR hunting studies in solid tumors, leading to the generation of a
TCR library for different antigens and HLA restrictions, thus rendering TCR gene editing an innovative off-the-shelf
treatment available for a high number of cancer patients. Awarding this fellowship will greatly enhance researcher’s career
not only by providing the opportunity to widen scientific knowhow and acquire new skills, but also by enabling the researcher
to address a major bottleneck currently limiting the full exploitation of the rapidly growing field of cancer immunotherapy.
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More information & hyperlinks
| Web resources: | https://cordis.europa.eu/project/id/752717 |
| Start date: | 01-01-2018 |
| End date: | 31-12-2019 |
| Total budget - Public funding: | 168 277,20 Euro - 168 277,00 Euro |
Cordis data
Original description
Recent encouraging clinical results obtained with engineered T lymphocytes and increasing advances in the genome editingfield, have opened new opportunities for T-cell receptor (TCR) gene therapy as an immunotherapeutic approach for cancer.
Unfortunately, the broad applicability of this treatment is still hampered by the possible mispairing of exogenous/endogenous
TCR chains and by the limited number of high avidity tumor-specific TCRs. While the first issue has been successfully
addressed by the hosting lab with the development a TCR gene editing protocol, the identification of novel tumor-specific
TCRs is urgently required and this is the aim of my research proposal. We have the unique opportunity to combine the highly
complementary expertise of the hosting lab in T-cell biology/genetic transfer and of the applicant on immune repertoire
sequencing. We will target acute myeloid leukemia (AML) and hypothesize that by exploiting intrinsic features of AML (i.e.
ability of AML blasts to differentiate into potent antigen presenting cells expressing tumor antigens), the functional fingerprint
induced by AML on tumor-reactive T-cells, and cutting-edge technologies (i.e. next generation sequencing; ligandome
landscape analysis), we will provide a comprehensive immunoprofiling of tumor-specific T-cells and isolate tumor TCR
specificities. Results obtained in this study will streamline TCR hunting studies in solid tumors, leading to the generation of a
TCR library for different antigens and HLA restrictions, thus rendering TCR gene editing an innovative off-the-shelf
treatment available for a high number of cancer patients. Awarding this fellowship will greatly enhance researcher’s career
not only by providing the opportunity to widen scientific knowhow and acquire new skills, but also by enabling the researcher
to address a major bottleneck currently limiting the full exploitation of the rapidly growing field of cancer immunotherapy.
Status
CLOSEDCall topic
MSCA-IF-2016Update Date
28-04-2024
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