AMNEsIA | Interactions of amyloid peptides with the neuronal membrane interface: molecular mechanisms involved in Alzheimer’s disease

Summary
The neuronal membrane is the target of specific molecular interactions involved in the development of Alzheimer’s disease (AD). The 2 hallmarks of the disease, extracellular amyloid β plaques and intracellular Tau neurofibrillatory tangles, are inherently linked to the membrane, as this biointerface induces and influences amyloid fibrillation. Moreover amyloid peptides selectively interact with the membrane depending of its composition, leading to toxicity mechanisms that are still poorly understood at the molecular scale. This project aims at providing a better understanding of the toxicity mechanisms of the amyloid peptides Aβ1-42 and Tau involved in AD on the neuronal membrane. Using Atomic Force Microscopy (AFM) with Carbon NanoTube (CNT) probes, we will obtain high-resolution imaging of these interactions at the nanometer scale. A unique functionalization of CNT tips with amyloid peptides will also allow true single molecule force spectroscopy of peptide/lipid interactions, unraveling their driving forces. With a progressive increase of the complexity in our system, we will, for the first time, investigate a potential synergy between Aβ1-42 and Tau and will develop an innovative approach to study live neurons by AFM imaging and force spectroscopy. Our study will establish new powerful methods to study the interactions of peptides with membranes or cells at the nanometer scale. We will not only contribute to the understanding of the deleterious effect of amyloid peptides on the neuronal membrane interface but also to the design of efficient preventive or therapeutic treatments, still lacking today.
Unfold all
/
Fold all
More information & hyperlinks
Web resources: https://cordis.europa.eu/project/id/794636
Start date: 01-09-2018
End date: 31-08-2020
Total budget - Public funding: 173 076,00 Euro - 173 076,00 Euro
Cordis data

Original description

The neuronal membrane is the target of specific molecular interactions involved in the development of Alzheimer’s disease (AD). The 2 hallmarks of the disease, extracellular amyloid β plaques and intracellular Tau neurofibrillatory tangles, are inherently linked to the membrane, as this biointerface induces and influences amyloid fibrillation. Moreover amyloid peptides selectively interact with the membrane depending of its composition, leading to toxicity mechanisms that are still poorly understood at the molecular scale. This project aims at providing a better understanding of the toxicity mechanisms of the amyloid peptides Aβ1-42 and Tau involved in AD on the neuronal membrane. Using Atomic Force Microscopy (AFM) with Carbon NanoTube (CNT) probes, we will obtain high-resolution imaging of these interactions at the nanometer scale. A unique functionalization of CNT tips with amyloid peptides will also allow true single molecule force spectroscopy of peptide/lipid interactions, unraveling their driving forces. With a progressive increase of the complexity in our system, we will, for the first time, investigate a potential synergy between Aβ1-42 and Tau and will develop an innovative approach to study live neurons by AFM imaging and force spectroscopy. Our study will establish new powerful methods to study the interactions of peptides with membranes or cells at the nanometer scale. We will not only contribute to the understanding of the deleterious effect of amyloid peptides on the neuronal membrane interface but also to the design of efficient preventive or therapeutic treatments, still lacking today.

Status

TERMINATED

Call topic

MSCA-IF-2017

Update Date

28-04-2024
Images
No images available.
Geographical location(s)
Structured mapping
Unfold all
/
Fold all
Horizon 2020
H2020-EU.1. EXCELLENT SCIENCE
H2020-EU.1.3. EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions (MSCA)
H2020-EU.1.3.2. Nurturing excellence by means of cross-border and cross-sector mobility
H2020-MSCA-IF-2017
MSCA-IF-2017