Summary
NaTOS: Nanoscale Thermo Optical Sensing is the concept of a new truly label free bio-sensor device capable of monitoring transient molecular interactions. The sensor utilizes plasmonic gold nanorods (NR) as both nanoscale heating source and optical near field sensor. Proteins and enzymes will be monitored as they change the intensity scattered by a single NR as they diffuse through the nanorod's nearfield.
The simultaneous laser induced and thus tunable heating of the NR will induces a nanoscale temperature gradient that allows to probe the analytes with respect to their thermophoretic behavior. This analyte specific behavior provides specific thermophortic fingerprints which will be used to identify and distinguish between different proteins and in further extend also to monitor the interaction between different species of analytes.
This novel type of sensor does not require fluorescent labels nor does it rely on tethering of specific receptors and the consequent binding of analytes to the NRs. In contrast to current state of the art techniques this truly label-free sensor does not suffer from binding induced life time restrictions and can obtain statistical significant datasets without the requirement, but with the possibility of multiplexing.
The simultaneous laser induced and thus tunable heating of the NR will induces a nanoscale temperature gradient that allows to probe the analytes with respect to their thermophoretic behavior. This analyte specific behavior provides specific thermophortic fingerprints which will be used to identify and distinguish between different proteins and in further extend also to monitor the interaction between different species of analytes.
This novel type of sensor does not require fluorescent labels nor does it rely on tethering of specific receptors and the consequent binding of analytes to the NRs. In contrast to current state of the art techniques this truly label-free sensor does not suffer from binding induced life time restrictions and can obtain statistical significant datasets without the requirement, but with the possibility of multiplexing.
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More information & hyperlinks
| Web resources: | https://cordis.europa.eu/project/id/792595 |
| Start date: | 01-03-2018 |
| End date: | 29-02-2020 |
| Total budget - Public funding: | 165 598,80 Euro - 165 598,00 Euro |
Cordis data
Original description
NaTOS: Nanoscale Thermo Optical Sensing is the concept of a new truly label free bio-sensor device capable of monitoring transient molecular interactions. The sensor utilizes plasmonic gold nanorods (NR) as both nanoscale heating source and optical near field sensor. Proteins and enzymes will be monitored as they change the intensity scattered by a single NR as they diffuse through the nanorod's nearfield.The simultaneous laser induced and thus tunable heating of the NR will induces a nanoscale temperature gradient that allows to probe the analytes with respect to their thermophoretic behavior. This analyte specific behavior provides specific thermophortic fingerprints which will be used to identify and distinguish between different proteins and in further extend also to monitor the interaction between different species of analytes.
This novel type of sensor does not require fluorescent labels nor does it rely on tethering of specific receptors and the consequent binding of analytes to the NRs. In contrast to current state of the art techniques this truly label-free sensor does not suffer from binding induced life time restrictions and can obtain statistical significant datasets without the requirement, but with the possibility of multiplexing.
Status
CLOSEDCall topic
MSCA-IF-2017Update Date
28-04-2024
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