Manuscript on novel 89Zr-GLP-1 peptide conjugation

An ARRIVE-compliant manuscript submitted for open-access publication describing the design and synthesis the GLP-1 site-specifically conjugated to the optimised 89Zr chelate, the design and synthesis the GLP-1 site-specifically conjugated to IRDye 800CW, molecular integrity and stability of GLP-1 conjugates analytically verified through LC-MS, in vitro evaluation of properties of 89Zr-labelled GLP-1 conjugate: chemical purity, stability, degradation, suitability for in vivo administration, in vivo evaluation of properties of 89Zr-labelled GLP-1 conjugate and IRDye 800CW GLP-1 conjugate Responsible for coordinating and assembling entire manuscript: DijonVerification: (1) PDF of manuscript; (2) Email from journal acknowledging receipt of manuscript; (3) Minutes of WP4 meeting prospectively justifying sample size for animal experiments; (4) A copy of the page on the TRISTAN data inventory (5.0.D1) which shows a record for the data acquired in order to generate this deliverable; (5) url of the site (5.0.D4) containing any SOPs and any exemplar data-sets associated with this deliverable.Responsible for mouse imaging: NijmegenResponsible for analytic verification: NijmegenGNG 4.1.G4: Thermodynamic and kinetic stability of 89Zr in these novel chelates in blood (in vitro) as well as physicochemical properties of labeled compounds should jusify further animal experiments Site-specific conjugated 89Zr-conjugated GLP-1 and the fluorescent analogue, in vitro are impressive enough to justify further animal experimentsVerification: Minutes of WP4 meetingConsequence: If conjugate is unimpressive, previously existing Zr chelates will be used for further studiesDate: M18