Select In vitro models of PNS-Tox

To obtain simplified highly predictive screening systems for highlighting neurotoxic compounds protocols and data obtained in vitro from rat cell cultures will be reported Neurons and glial cells cultures will allow to obtain models of neurotoxicity evoked by the selected drugs Measurements of drugs concentration in cell media and in the cytosolic fraction will be reported to individuate a parallelism with plasma and tissue toxic concentration A validation of these methods will be obtained by reproducing the same treatments in differentiated human induced pluripotent stem cells hiPSCs preparations Data about cell viability apoptotic process intracellular organelles functionality transcriptomic and proteomic analysis will be summarized