Simple, fast and efficient iTOP-mediated delivery of CRISPR/Cas9 RNP in difficult-to-transduce human cells including primary T cells

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Authors: Waleed M Kholosy, Marieke Visscher, Kim Ogink, Helen Buttstedt, Kelli Griffin, Axel Beier, Jan Patrick Gerlach, Jan J Molenaar, Niels Geijsen, Marco de Boer, Anna Chatsisvili

Journal title: Journal of Biotechnology

Journal number: 338

Journal publisher: Elsevier BV

Published year: 2021

Published pages: 71-80

DOI identifier: 10.1016/j.jbiotec.2021.07.006

ISSN: 0168-1656