Summary
We will generate a novel antibody panel containing markers for candidate proteins with variable expression in subtypes of the known pancreas cells and validate it for usage in freshly frozen and FFPE pancreas samplesWe will use the HPA resource to create novel validated antibody panels for subsequent high parametric analysis of protein expression in the pancreas using the CODEX technology The antibody panel will be generated and validated for usage in freshly frozen and FFPE pancreas samples and contain the following a markers for known cell types in pancreas D44 b candidate proteins with variable expression in subtypes of the known pancreas cells D45 c a proliferation marker and d markers for cellular organelles plasma membrane Golgi apparatus mitochondria nucleus to allow for cell segmentation and highdefinition phenotypic analysis
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