LOC for single cell capture and lysis

LOC for single cell capture and lysis DTU will develop an opto-fluidic chip able to trap, visualise and lyse individual cells and separate metaphase chromosomes from cell lysate. The chip represents the other upstream element of the integrated device that is going to deliver chromosomes to the CTFM-3DSR instrument (see task 2.4). The microfluidic element for sample preparation – single cell capture, visualisation, lysing and chromosome sample cleaning – will be developed with three approaches in parallel: (1) Single cells are captured in parallel flow constrictions; (2) in 2-beam optical traps (stretchers), integrated on the plastic chip; (3) in a hydrogel turned on and off by a near-infrared laser. The captured cells are then visualized and lysed by buffer exchange. This will lead to a decision point at M24, which approach to use in the further developments. UCPH will use the devices as standalone tools for biological investigations (tasks 2.5).