Real-time data of UFB formation and resolution

Real-time data of UFB formation and resolution The VU and UCPH groups will investigate whether UFBs can be visualised in whole metaphase chromosomes following the application of force to both sister chromatids by our optical tweezers. UFBs will be visualised using purified PICH-GFP protein. A question we aim to answer is whether PICH can act on its own or requires co-factors to access UFBs. This task will demonstrate our ability to access specific processes that take place on chromosomes and therefore acts as a roadmap for further fundamental science questions (as for exampled explored in task 1.6) that can be pursued with the instrument.