Validation CTFM-SR3D in biological/medical setting

Validation CTFM-SR3D in biological/medical setting. UCPH and UCL scientists will be trained to work with the second CTFM-SR3D instrument, first at LUMICKS and later at their institutes to carry-out the following experiments (in order of complexity): (1) Isolate meiotic chromosomes from normal and disease conditions to analyse the structure of recombination–driven paired homologous chromosomes. Can we discover why women over 35 years have an exponential increase in chromosome nondisjunction leading to aneuploidy in ova? (2) Develop tools for diagnosis of ‘chromosome diseases’ such as cancer/neurological disorders. This might entail generation of probes/paints that specifically recognise each chromosome to study the formation of chromosome translocations, chromosome fusions and aneuploidy in an automated way. This could develop into high throughput karyotyping in the future, a currently difficult and laborious procedure (3) Can we reconstitute the ‘search for homology’ between 2 recombining sequences placed on different chromosomes or between 2 homologous chromosomes?