Report on transposon for converting any Gram-negative bacteria in an HFR strain

This will involve a transposon able to mobilize an entire chromosome though conjugation between a donor and a DNAless recipient For this all the mobtra genes involved in conjugal transfer of superpromiscuous plasmid RP4 will be retrieved from their original genetic frame and assembled as an artificial gene cluster in a miniTn5 transposon vector Upon aquisition of such a transposon any strain will acquire the ability of selftransference and therefore will behave as an HFR strain Highfrequency of recombination that will ease the passing of the genomic information among strains with different degrees of NTN reaction evolution