Report on acoustic signal enhancement during ctDNA detection with lossy particles

Particles identified in WP2 to have a high dissipative capacity will be tested for the detection of spiked synthetic oligos carrying the mutations of interest in serum in combination with the optimized protocol (Task 3.3). The non-specific binding of the particles to the device surface will be studied and eliminated if present; initial results obtained by P1 showed zero liposomes’ binding on a neutravidin-surface modified with DNAs. Finally, micro-devices optimized in WP1 with respect to their frequency will be used in these studies to select the most sensitive combination that will give the anticipated 103 (aM) to 106-fold (zM) signal enhancement.