Targeted NGS analysis on the tissue samples of the 200 patients

In the case of solid tumours, formalin fixed paraffin embedded (FFPE) blocks are usually the only tumor material available. Although the DNA obtained from FFPE is highly fragmented and cross-linked due to fixation, it has been demonstrated to yield NGS results comparable to those of fresh frozen tissue, as long as appropriate protocols are employed.21 Based on its long experience, the Lab. of Tumor Biology at the UOC will ensure robust analysis of the DNA from FFPE blocks. The mutations identified by NGS will also be confirmed by alternative methods depending on the type of alteration to be validated. P4 is in close collaboration with the Medical Oncology Dept., Univ. Hospital of Heraklion and runs mutation tests for diagnostic purposes in more than 200 samples per year.