Robust microfabrication protocols to embed hydrophobic fluoropolymers membranes within microfluidic chips

Report on the development of fabrication protocols to embed the hydrophobic fluoropolymers membranes developed in WP3 within custom-made microfluidic devices obtained by standard PDMS soft lithography. These protocols may use mechanical clamping to seal the membranes between two micro-fabricated PDMS stamps or chemical linking with slight chemical modifications of the PDMS surface (preserving the requirements for mAbs crystallization).