Standardized SOPs for the optimized isolation of different cell types

This deliverable will be acheived by : Task 2.1 - optimization of primary and stem cell isolation protocols from tissues (e.g. adipose derived stem cells, cardiomyocytes, chondrocytes, osteoblasts, hepatocytes, pancreatic islets and fibroblasts) using formulations of specific collagenase class I/II ratios and of protease amount/type (thermolysin, dispase, pronase); Task 2.2 – evaluation of new biomarine enzyme formulations currently under development at Abiel; Task 2.3 – assessment of the reproducibility of results (e.g. cell yield, viability and functionality) and comparison to current standard isolation procedures (e.g. via commercial competitor products).