Histone methylation and DNAm profiles of the HIV-1 promoter ; new druggable targets within HMTs, HDMs and DNMTs; Test and select drugs to be validated in WP5

Compare the histone methylation status of the HIV promoter following siRNA-mediated depletion of HMTs or HDMs using in vitro HIV-1 latency cellular models. Compare the HIV promoter DNA methylation status by the bisulfite conversion sequencing following DNMTs depletion using in vitro HIV-1 latency cellular models. Compare the HIV promoter methylation status in distinct memory CD4+ T cells subsets latently infected with HIV in patient under suppressive cART. Analyze HMTs/HDMs recruitment at the HIV-1 promoter in vivoby chromatin immunoprecipitation (ChIP)-qPCR using in vitro HIV-1 latency cellular models. Compare HIV-1 proviral gene expression and production following siRNA-mediated DNMTs knockdown using in vitro HIV-1 latency cellular models. Test and select new latency-reversible compounds targeting functionally validated HMTs, HDMs or DNMTs using in vitro HIV-1 latency cellular models.