Determination of the potency, synergistic activity and toxicity of novel classes of LRA

HMTI, HDACI, HDMT inhibitors, DNA methylation inhibitors, NF-ĸB inducers and compounds releasing active P-TEFb complex will be tested first alone and in combination in latently-infected cell lines. Analyze the effect of the HIV-1 inducers on viral reactivation in ex vivo cultures of CD8+-depleted PBMCs. We will also study the patient-specific variations in the ex vivo reactivation capacity of the HIV-infected cells in ex vivo cultures of resting CD4+ T cells. Resting (CD69- CD25- HLA-DR-)