Quantitative analyses of local polarity perturbations on polarized growth

Quantitative analyses of local polarity perturbations on polarized growth will involve a) generation of strains which can generate locally increased concentrations of PS, PIP2, activated Cdc42 and activated Rho1, b) Quantitative analyses of local perturbation of PS, PIP2, activated Cdc42 and activated Rho1 levels on external mediated polarized growth and force generation.