Mechanistic role of microtubules in polarization of 3D-cultured epithelial cells

Elucidating the mechanistic role of microtubules in polarization of 3D-cultured epithelial cells involves a) quantitative description of the role of microtubule-attachment complexes in cortical polarization in 3D breast epithelia acini and their modification during EMT and invasive growth in 3D, b) characterization of the biochemical connection between microtubule attachment complexes and the Scrib complex, c) quantitative characterization of the role of microtubule dynamics in polarity maintenance in 3D-cultured epithelial cells and the effects of microtubule-targeting agents on this process.