SOP for pre-analytics in blood assays

The pre-analytical stability of the novel biomarker tests need to be intensively studied. Pre-analytical SOPs have been standardised for CSF analysis32. Due to the higher cellular and protein contents relative to CSF, blood biomarker levels are strongly affected by differences in preanalytical protocols. Moreover, besides demographic factors (age, gender), lifestyle factors such as fasting status, excercise, can influence the results. Thus, for blood analysis of neurospecific proteins, and specifically for the targetted proteins, such SOPs need to be developed in order to get accurate and reproducible results, and studies to address all these pre-analytical factors should be design to provide a rationale for such SOPs.