CRISPR/Cas9 gRNA activity depends on free energy changes and on the target PAM context

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Authors: Giulia I. Corsi, Kunli Qu, Ferhat Alkan, Xiaoguang Pan, Yonglun Luo, Jan Gorodkin

Journal title: Nature Communications

Journal number: 13

Journal publisher: Nature Publishing Group

Published year: 2022

Published pages: Article number: 3006

DOI identifier: 10.1038/s41467-022-30515-0

ISSN: 2041-1723