Demonstration-scale Chlorella production unit

To achieve a demonstration-scale Chlorella production unit, different systems will be installed in the winery site, including: 1) water collection, treatment and storage system; 2) cultivation medium preparation and storage system; 3) carbonation system (a pure CO2 supply system will be installed as risk mitigation strategy for fermentation off-gas supply); 4) inoculum production systems - 7 and 30m2 unilayer horizontal tubular PBRs (A4F); 5) demo-scale cultivation systems - 150m2 RW(COLD) and 115m2 unilayer horizontal tubular PBR (A4F); 6) temperature regulation system; 7) harvesting system - air flotation (COLD), centrifuge and/or membrane filtration (A4F/LEITAT); 8) downstream processing system - cell disruption (bead mill), centrifugation (disc stack) and ultrafiltration modules; 9) monitoring, automation and control system. When in operation, such unit will obtain Chlorella biomass, as well as Chlorella bioactive ingredients. A4F will develop the engineering project and will execute the civil engineering work necessary and the installation of the unit, in collaboration with COLD, for cultivation and harvesting, and AVIPE, whose one of the associates will host the unit.